The California National Primate Research Center (CNPRC) maintains a colony of approximately 5000 rhesus macaques (Macaca mulatta) housed in outdoor half acre corrals, smaller outdoor “corn cribs,” indoor caging, and indoor nurseries. Each year, the majority of available 3-to-4-month-old infants in the colony participate in a 25-h Biobehavioral Assessment (BBA) program designed to characterize behavioral and physiological responsiveness in young animals. More than 2000 infants have been tested since 2001. An electronic database has been prepared with data from the BBA.
The procedures used to generate the BBA data used in this report have been described previously (Capitanio et al., 2005 (link); Capitanio et al., 2006 ; Golub et al., 2006 (link)). Infants between 90 and 120 days of age were separated from their dams and relocated from their home cages to individual indoor cages (Holding Cage, 60 × 65 × 79 cm, Lab Products, Inc., Maywood, NJ) in the morning on the day of testing. Five to 8 infants, comprising a single cohort, were tested at the same time. The Holding Cage contained a cloth diaper, a stuffed terry cloth duck and a novel manipulable object (approximately 4 × 9 cm) containing activity sensors. Infants were provided with water, a fruit-flavored drink, commercial monkey diet and fresh fruit.
The commercial diet fed to the monkeys in outdoor corrals and during BBA participation (Lab Diet #5038, Purina Mills International, St. Louis MO) contained 230 ppm iron as iron carbonate. Infants were tested in the same predetermined random order for each test throughout the 25-h period (Table 1 ). For most assessments, they were transferred to a test cage in an adjacent room and videotaped. Tapes were later scored using The Observer software (Noldus Information Technology) for frequency and duration of behaviors. Plasma cortisol was measured in 4 blood samples taken during the 25-h period (see below). At the conclusion of testing on the second day, infants were returned to their mothers and released back to their home enclosures. Behavioral data obtained by observers at the time of the BBA and video-recorded human intruder data were available for this study. Data from the other videorecorded tests (preferential look, video playback of response to social stimuli) have not yet been scored for all animals. Intra- and inter-observer reliabilities are checked annually, and exceed 85% agreement.
The procedures used to generate the BBA data used in this report have been described previously (Capitanio et al., 2005 (link); Capitanio et al., 2006 ; Golub et al., 2006 (link)). Infants between 90 and 120 days of age were separated from their dams and relocated from their home cages to individual indoor cages (Holding Cage, 60 × 65 × 79 cm, Lab Products, Inc., Maywood, NJ) in the morning on the day of testing. Five to 8 infants, comprising a single cohort, were tested at the same time. The Holding Cage contained a cloth diaper, a stuffed terry cloth duck and a novel manipulable object (approximately 4 × 9 cm) containing activity sensors. Infants were provided with water, a fruit-flavored drink, commercial monkey diet and fresh fruit.
The commercial diet fed to the monkeys in outdoor corrals and during BBA participation (Lab Diet #5038, Purina Mills International, St. Louis MO) contained 230 ppm iron as iron carbonate. Infants were tested in the same predetermined random order for each test throughout the 25-h period (
