Drosophila Embryogenesis and Larval Development

Drosophila melanogaster were reared in cages at 25°C, unless indicated otherwise. Embryos were collected onto freshly yeasted apple juice plates overnight and staged according to Campos-Ortega and Hartenstein (1985) . For larval experiments, larvae were picked within 1 h of hatching [designated 0 h after larval hatching (ALH)], transferred to a yeasted food plate and reared to the desired stage before dissection.
The following stocks were used: w¹¹¹⁸, GAL4^c855a (Manseau et al., 1997 (link)), R9D11-mCD8-GFP (Zhu et al., 2011 (link)), R9D11-CD4-tdTomato (Han et al., 2011 (link)), (miR-7)E>GFP (Li et al., 2009 (link)), wg-LacZ (1-en-11) (Kassis et al., 1992), hh^P30 (Lee et al., 1992 (link)) and HLHmγ-GFP (Almeida and Bray, 2005 (link)). The following stocks were obtained from the Bloomington Drosophila Stock Center: dpp-lacZ^Exel.2 (#8411), UAS-myr-mRFP (#7119), R31H09-GAL4 (#49694), R29C07-GAL4 (‘ogre-GAL4’, #49340) and pnt-GFP.FPTB (#42680). To perform FLEXAMP, we crossed flies carrying yw; tub-Gal80^ts, UAS-flp; act>y⁺>LHV2^deltaRFP-86Fb (LexA) (Yagi et al., 2010 (link)) to flies carrying 13XLexAOp2-mCD8-GFP (Bloomington #32205), R31H09-GAL4 and tub-GAL80^ts (Bloomington #7019).

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Hakes A.E., Otsuki L, & Brand A.H. (2018). A newly discovered neural stem cell population is generated by the optic lobe neuroepithelium during embryogenesis in Drosophila melanogaster. Development (Cambridge, England), 145(18), dev166207.

Publication 2018

UAS-myr-mRFP R31H09-GAL4 13XLexAOp2-mCD8-GFP tub-GAL80ts

Dissection Drosophila Drosophila melanogaster Embryos Flies Food Lacz Larval Mir 7 Tdtomato

Corresponding Organization :

Other organizations : Wellcome/Cancer Research UK Gurdon Institute, University of Cambridge

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Variable analysis

independent variables

Temperature (25°C)

dependent variables

Embryo development and staging
Larval development and stage

control variables

Drosophila melanogaster reared in cages
Embryos collected onto freshly yeasted apple juice plates overnight
Larvae picked within 1 h of hatching and transferred to a yeasted food plate

controls

Positive controls: w^1118, GAL4^c855a, R9D11-mCD8-GFP, R9D11-CD4-tdTomato, (miR-7)E>GFP, wg-LacZ (1-en-11), hh^P30, HLHmγ-GFP, dpp-lacZ^Exel.2, UAS-myr-mRFP, R31H09-GAL4, R29C07-GAL4 ('ogre-GAL4'), pnt-GFP.FPTB
Negative control: tub-Gal80^ts

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