A total of 302 non-duplicate E. faecalis isolates were collected from different inpatients at Shenzhen Nanshan People’s Hospital (Grade A, level III Hospital, 1500 beds), Shenzhen University, China, between January 1, 2011, and December 31, 2016. These E. faecalis isolates were obtained from urine (135 isolates), blood (37 isolates), pus or secretions (86 isolates), bile (25 isolates), and other clinical sources (19 isolates). Based on a previous study, the dominant multilocus sequence types (MLSTs) of these isolates were ST16 and ST179 (Zheng et al., 2017 (link)). The isolates were identified by the Phoenix 100 automated microbiology system (BD, Franklin Lakes, NJ, United States), following which two subcultured generations of all the 302 isolates were re-identified with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (IVD MALDI Biotyper, Bruker, Bremen, Germany). E. faecalis strains ATCC29212 and OG1RF (ATCC47077) were used as reference strains.
Chloramphenicol (catalog no. HY-B0239), linezolid (catalog no. HY-10394), and radezolid (catalog no. HY-14800) were purchased from MedChemExpress (MCE, Shanghai, China).
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