Comprehensive Drosophila Genetics Toolkit

FRT19A, fzr^A (#52384), FRT19A, fzr^B (#52385), and Dp(1;3)DC120 (#30265) were obtained from BDSC. FRT19A, fzr^G0418 (#111943) was obtained from Kyoto Stock Center. UAS-Fzr-HA (#F000893) was obtained from FlyORF. UAS-HA-Rca1 is a gift from Dr. Frank Sprenger (Grosskortenhaus and Sprenger, 2002 (link)) and FRT19A, fzr^8F3 (P{neoFRT}19A/FM7) was a gift from Dr. Christian Klambt. EGFP-Fzr^BAC was generated in this study.
RNAi lines used in this study: erm_RNAi (BDSC #26778), fzr_RNAi (GD#25553), cdc20_RNAi_1 (GD#40500), cdc20_RNAi_2 (GD#44834), ida_RNAi (BDSC#34522); brm_RNAi (GD#37720), β-gal_RNAi (BDSC#50680), and bcd_RNAi (GD#48966).
Neural stem cell drivers included insc-Gal4 (BDSC#8751; 1407-Gal4) or wor-Gal4 (BDSC#56553). Glial driver was repo-Gal4 (BDSC#7415). Type I NSC driver (ase-Gal4; UAS-mCD8-GFP, T. Lee). Type II NSC driver (w; UAS-Dicer2, wor-Gal4, ase-Gal80; UAS-mCD8-GFP) (Bowman et al., 2008 (link)). INP driver (erm-Gal4/CyO) (Pfeiffer et al., 2008 (link)). Other drivers used in this study are: nerfin-1-Gal4, UAS-mCD8-GFP (Louis Y. Cheng), pros-Gal4 (BDSC#80572), and elav-Gal4 (BDSC#458). UAS-Dcr2 (BDSC#24650) or/and UAS-CD8-GFP (BDSC#32186) was used together with various Gal4 drivers or RNAi stocks.
Experiments with mutants were performed at 25°C, and experiments for RNAi-mediated knockdown or overexpression were carried out at 29°C.

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Ly P.T, & Wang H. (2020). Fzr/Cdh1 Promotes the Differentiation of Neural Stem Cell Lineages in Drosophila. Frontiers in Cell and Developmental Biology, 8, 60.

Publication 2020

FRT19A repo-Gal4 UAS-mCD8-GFP elav-Gal4 UAS-Dcr2

Dc120 Dcr2 Glial Neural stem cell Pros Rnai

Corresponding Organization : National University of Singapore

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Variable analysis

independent variables

FRT19A, fzrA (#52384)
FRT19A, fzrB (#52385)
Dp(1;3)DC120 (#30265)
FRT19A, fzrG0418 (#111943)
UAS-Fzr-HA (#F000893)
UAS-HA-Rca1
FRT19A, fzr8F3 (P{neoFRT}19A/FM7)
EGFP-FzrBAC

dependent variables

Measured outcomes not explicitly mentioned

control variables

Experiments with mutants were performed at 25°C
Experiments for RNAi-mediated knockdown or overexpression were carried out at 29°C

positive controls

None specified

negative controls

None specified

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