Antibodies for Detecting CIRP, RBM3, and Related Proteins

Rabbit polyclonal antibodies recognizing the C terminus of mouse CIRP and mouse RBM3 were prepared as described^{7 (link), 8 (link)}. The sources of commercial antibodies were as follows: anti-ACTIN (mouse monoclonal, clone C4, Millipore), anti-BAX (rabbit polyclonal, #2772, Cell Signaling), anti-SRSF1/SF2/ASF (mouse monoclonal, sc-33652, Santa Cruz Biotechnology, Inc., Texas), anti-SRSF2/SC35 (goat polyclonal, sc-10252, Santa Cruz), anti-SRSF5/SRp40 (rabbit polyclonal, Sigma-Aldrich; rabbit polyclonal, MBL, Nagoya, Japan), anti-SRSF6 (rabbit polyclonal, Sigma-Aldrich), anti-p53 (rabbit polyclonal, sc-6243, Santa Cruz Biotechnology), anti-p73 (rabbit monoclonal, ab40658, Cambridge, UK), anti-mouse immunoglobulins/HRP (goat polyclonal, DakoCytomation, Glostrup, Denmark), anti-rabbit immunoglobulins/HRP (goat polyclonal, DakoCytomation), and FITC-conjugated goat anti-rabbit immunoglobulins (DakoCytomation).

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Fujita T., Higashitsuji H., Higashitsuji H., Liu Y., Itoh K., Sakurai T., Kojima T., Kandori S., Nishiyama H., Fukumoto M., Fukumoto M., Shibasaki K, & Fujita J. (2017). TRPV4-dependent induction of a novel mammalian cold-inducible protein SRSF5 as well as CIRP and RBM3. Scientific Reports, 7, 2295.

Publication 2017

anti-ACTIN anti-BAX anti-p53 anti-mouse immunoglobulins/HRP anti-rabbit immunoglobulins/HRP FITC-conjugated goat anti-rabbit immunoglobulins

Actin Anti immunoglobulins Antibodies Clone Fitc Goat Mouse Rabbit

Corresponding Organization :

Other organizations : Kyoto University, Kindai University, University of Tsukuba, Tohoku University, Gunma University

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Variable analysis

independent variables

Rabbit polyclonal antibodies recognizing the C terminus of mouse CIRP
Rabbit polyclonal antibodies recognizing the C terminus of mouse RBM3

dependent variables

Not explicitly mentioned

control variables

Anti-ACTIN (mouse monoclonal, clone C4, Millipore)
Anti-BAX (rabbit polyclonal, #2772, Cell Signaling)
Anti-SRSF1/SF2/ASF (mouse monoclonal, sc-33652, Santa Cruz Biotechnology, Inc., Texas)
Anti-SRSF2/SC35 (goat polyclonal, sc-10252, Santa Cruz)
Anti-SRSF5/SRp40 (rabbit polyclonal, Sigma-Aldrich; rabbit polyclonal, MBL, Nagoya, Japan)
Anti-SRSF6 (rabbit polyclonal, Sigma-Aldrich)
Anti-p53 (rabbit polyclonal, sc-6243, Santa Cruz Biotechnology)
Anti-p73 (rabbit monoclonal, ab40658, Cambridge, UK)
Anti-mouse immunoglobulins/HRP (goat polyclonal, DakoCytomation, Glostrup, Denmark)
Anti-rabbit immunoglobulins/HRP (goat polyclonal, DakoCytomation)
FITC-conjugated goat anti-rabbit immunoglobulins (DakoCytomation)

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