Alloxan-Induced Diabetes in Mice

Diabetes mellitus was induced by intravenous injection of alloxan monohydrate (50 mg/kg; Sigma Chemical Co., St. Louis, MO, USA) dissolved in physiologic saline (SAL, 0.9% NaCl). Control mice were injected with physiologic SAL only. After 10 days, the presence of diabetes was verified by blood glucose concentrations higher than 300 mg/dL, which were determined with a blood glucose monitor (Accu-Chek Advantage II, Roche Diagnostica, São Paulo, São Paulo, Brazil), in blood samples obtained from mouse tails (15 (link)).

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Ferreira S.S., Nunes F.P., Casagrande F.B, & Martins J.O. (2017). Insulin Modulates Cytokine Release, Collagen and Mucus Secretion in Lung Remodeling of Allergic Diabetic Mice. Frontiers in Immunology, 8, 633.

Publication 2017

alloxan monohydrate Accu-Chek Advantage II

0 9 nacl Alloxan Blood Blood glucose Diabetes Mouse Physiologic Saline Tails

Corresponding Organization : Universidade de São Paulo

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Variable analysis

independent variables

Injection of alloxan monohydrate (50 mg/kg)

dependent variables

Blood glucose concentrations

control variables

Injection of physiologic saline (0.9% NaCl)

controls

Injection of physiologic saline (0.9% NaCl)

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