The cells were plated in 96-well plates (3–5 × 103/well) for 12 h and were treated as described before [42 (link)]. Then, cells were incubated with 20 μL of 5 mg/mL MTT (Abcam, Cambridge, UK) for 2 h and the resulting formazan crystals were dissolved in dimethyl sulfoxide (200 μL). Absorbance was measured at 490 nm using the Thermo Scientific™ Multiskan™ (Waltham, MA, USA) FC Microplate Photometer. All the data were normalized with vehicle-treated control cells. The experiment was performed at least in triplicate and repeated three independent times.
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