Mice were anesthetized, and then blood was obtained via the orbital sinus and was collected in anticoagulant tubes. The numbers of peripheral blood cells included white blood cells (WBCs), red blood cells, platelets, and the hemoglobin (HGB) were measured by an MEK-7222 K (Nihonkohden, Japan). BM cells were harvested as previously described [25 (link)]. The number of viable BMNCs was counted and expressed as ×107/femur.
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