The MDA-MB-231 breast cancer cell line was from the American Type Culture Collection (ATCC, Manassas, VA) and maintained as described [27] (link). The identity of the line was confirmed by The Research Animal Diagnostic Laboratory (RADIL) at the University of Missouri, Columbia, MO (http://www.radil.missouri.edu), using a PCR based method that detects 9 short tandem repeat (STR) loci, followed by comparison of results to the ATCC STR database. A cell bank of defined passage was established and cells were propagated for no more than ten passages in culture.
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