Twenty-five kilograms of fresh tea leaves of the Camellia sinensis (Jin Guanyin) variety (one bud with two or three leaves) were collected from the tea garden at Qilin Mountain Tea Factory, Fujian, China. These tea leaves were then divided into five portions for the manufacturing of DL, GT, OT, BT and WT at the tea factory of Fujian Agriculture and Forestry University by the manufacturing procedures illustrated in Figure S1 .
For cell culture experimentation, 25 g of dried tea powder were extracted with water for 30 min at 80 °C and then freeze-dried into powder after filtering. The tea extracts were stored at −80 °C and diluted by PBS until use.
Chemical profiling analysis was performed according to previous methods [49 (link),50 (link)]. Briefly, freeze-dried tea leaves were individually ground to fine powders using precooled mortar and pestle. Following lyophilization, 30 mg (±0.5 mg) of ground samples were weighed and 1.2 mL of 70% (v/v) methanol was added for metabolite extraction. Samples were vortexed, sonicated at 25 °C for 20 min and centrifuged (10 min, 12,000 g). Supernatants were diluted 50-fold with 70% (v/v) methanol, filtered through a 0.22 µm PVDF filter (Millipore) and stored at −20 °C until analyzed. Three biological sample replicates were prepared for each tea types.
For cell culture experimentation, 25 g of dried tea powder were extracted with water for 30 min at 80 °C and then freeze-dried into powder after filtering. The tea extracts were stored at −80 °C and diluted by PBS until use.
Chemical profiling analysis was performed according to previous methods [49 (link),50 (link)]. Briefly, freeze-dried tea leaves were individually ground to fine powders using precooled mortar and pestle. Following lyophilization, 30 mg (±0.5 mg) of ground samples were weighed and 1.2 mL of 70% (v/v) methanol was added for metabolite extraction. Samples were vortexed, sonicated at 25 °C for 20 min and centrifuged (10 min, 12,000 g). Supernatants were diluted 50-fold with 70% (v/v) methanol, filtered through a 0.22 µm PVDF filter (Millipore) and stored at −20 °C until analyzed. Three biological sample replicates were prepared for each tea types.
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