Targeting the HER3 signaling pathway

The anti-HER3 antibody 9F7-F11 has been described elsewhere [2 (link), 32 (link), 33 (link), 36 (link)]. Rabbit monoclonal antibodies against HER3 and phosphorylated (p) HER3 (Tyr1289), PARP (clone 46D11), cleaved caspase-8 (Asp391; clone 18C8), caspase-9 and cleaved caspase-9, caspase-3 (clone 8G10) and cleaved caspase-3, XIAP, DR5 (clone D4E7), BIM, c-FLIP (clone D16A8), USP9X, β-actin and β-tubulin were from Cell Signaling Technology (Danvers, MA). The mouse monoclonal antibody against ITCH was from BD Biosciences (San José, CA; ref.611199). The rabbit polyclonal antibodies against HER3 (clone C17), USP8, USP9X, c-FLIP_L/S (clone H-202), BAX, FAS, TRAIL, DcR2, cIAP2, and BID were from Santa Cruz Biotechnology (Santa Cruz, CA). For detection of activated ITCH, a rabbit anti-pITCH (Thr222) antibody from Millipore (Billerica, MA) was used. The human recombinant NRG1-β1 extracellular domain (ECD) was from RD Systems (Minneapolis, MN) and was used at 100 ng/ml. The proteasome inhibitor MG132 and chlorimipramine (CI) were from Sigma-Aldrich (St Louis, MO). The control IgG antibody used for co-immunoprecipitation experiments was from Santa Cruz Biotechnology.

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Le Clorennec C., Lazrek Y., Dubreuil O., Sampaio C., Larbouret C., Lanotte R., Poul M.A., Barret J.M., Prost J.F., Pèlegrin A, & Chardès T. (2019). ITCH-dependent proteasomal degradation of c-FLIP induced by the anti-HER3 antibody 9F7-F11 promotes DR5/caspase 8-mediated apoptosis of tumor cells. Cell Communication and Signaling : CCS, 17, 106.

Publication 2019

Rabbit monoclonal antibodies β-actin β-tubulin cIAP2 MG132 control IgG antibody

Actin Anti antibody Antibodies Antibody Caspase 3 Caspase 8 Caspase 9 Chlorimipramine Ciap2 Clone Co immunoprecipitation Dcr2 Her3 Human Igg antibody Itch Mg132 Monoclonal antibody Mouse Nrg1 Proteasome inhibitor Rabbit Trail Tubulin Usp8 Usp9x

Corresponding Organization : Centre de Gestion Scientifique

Other organizations : Inserm, Université de Montpellier, Institut de Recherche en Cancérologie de Montpellier, Université Bourgogne Franche-Comté

Top 5 similar protocols

Variable analysis

independent variables

Anti-HER3 antibody 9F7-F11
Proteasome inhibitor MG132
Chlorimipramine (CI)

dependent variables

Phosphorylated (p) HER3 (Tyr1289)
PARP (cleaved)
Caspase-8 (cleaved)
Caspase-9 (cleaved)
Caspase-3 (cleaved)
C-FLIP
USP9X
ITCH (activated)

control variables

Rabbit monoclonal antibodies against HER3 and phosphorylated (p) HER3 (Tyr1289), PARP (clone 46D11), cleaved caspase-8 (Asp391; clone 18C8), caspase-9 and cleaved caspase-9, caspase-3 (clone 8G10) and cleaved caspase-3, XIAP, DR5 (clone D4E7), BIM, c-FLIP (clone D16A8), USP9X, β-actin and β-tubulin
Mouse monoclonal antibody against ITCH
Rabbit polyclonal antibodies against HER3 (clone C17), USP8, USP9X, c-FLIP_L/S (clone H-202), BAX, FAS, TRAIL, DcR2, cIAP2, and BID
Rabbit anti-pITCH (Thr222) antibody
Human recombinant NRG1-β1 extracellular domain (ECD)
Control IgG antibody for co-immunoprecipitation experiments

positive controls

Human recombinant NRG1-β1 extracellular domain (ECD) used at 100 ng/ml

negative controls

Control IgG antibody used for co-immunoprecipitation experiments

Annotations

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