The cell lines CWR-R1-enzalutamide resistant/luciferase+ (stably expressing a firefly luciferase), and CWR-R1-enzalutamide resistant/luciferase plus FAP (additionally expressing a human FAP receptor) were provided by the LeBeau lab from the University of Wisconsin (28 (link)). Both cell lines and 293AAV cells (Cell Biolabs) were cultured in DMEM (Gibco) supplemented with 10 % fetal bovine serum (Gibco), 4.5 g/L D-glucose, L-glutamine, 110 mg/L sodium pyruvate, and 100 U per mL penicillin/100 μg per mL streptomycin (Gibco). Media for R1-FAP cells was additionally supplemented with 3 μg/mL puromycin (ApexBio Technology). Cells were kept in a humidified incubator at 37 °C and 5 % CO2 and passaged every 2-4 days when reaching a confluency of 70-80 %.
Protocol detail
Culturing Enzalutamide-Resistant Prostate Cancer Cell Lines
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Corresponding Organization :
Other organizations : University of Minnesota System, University of Wisconsin–Madison
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Variable analysis
independent variables
- Cell lines: CWR-R1-enzalutamide resistant/luciferase+ and CWR-R1-enzalutamide resistant/luciferase plus FAP
- Not explicitly mentioned
- Cell culture conditions: DMEM supplemented with 10% fetal bovine serum, 4.5 g/L D-glucose, L-glutamine, 110 mg/L sodium pyruvate, and 100 U per mL penicillin/100 μg per mL streptomycin
- Incubator conditions: Humidified, 37 °C, 5% CO2
- Passage frequency: Every 2-4 days when reaching 70-80% confluency
- Positive control: 293AAV cells
- Negative control: Not explicitly mentioned
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