One hundred milligram Dex powder (Solarbio, Beijing, China) was dissolved in 1 mL dimethyl sulfoxide, and mixed well, and 19.62 μL of Dex solution was aspirated and added to 10% v/v sodium pentobarbital (FBS) low sugar dulbecco's modified eagle's medium (DMEM) culture solution to fix the volume to 50 mL to obtain a concentration of 3.924 6 × 10−4 g/L of dexamethasone sodium phosphate preparation solution. Short‐term refrigerated storage at 4°C, long‐term frozen storage at −20°C.
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Dexamethasone Sodium Phosphate Preparation
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Corresponding Organization :
Other organizations : Shandong University of Traditional Chinese Medicine, Affiliated Hospital of Shandong University of Traditional Chinese Medicine
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Variable analysis
independent variables
- Dex powder (Solarbio, Beijing, China) concentration
- Dexamethasone sodium phosphate preparation solution concentration
- Solvent: dimethyl sulfoxide
- Culture solution: 10% v/v sodium pentobarbital (FBS) low sugar dulbecco's modified eagle's medium (DMEM)
- Storage conditions: short-term refrigerated storage at 4°C, long-term frozen storage at -20°C
- No positive or negative controls were explicitly mentioned.
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