Investigating Triazol-based Compounds for Research

ICZ (cis-4[4-4-4[[2-(2-4-dichlorophenyl)-2-(1H-1,2,4,triazol-1-methyl)-1,3-dioxolan-4-yl]-1-piperazinyl]phenyl]-2,4-dihydro-2-(1-methyl-propyl)-3H-1,2,4-triazol-3-one; MW: 705.64) and nocodazole were purchased from Sigma-Aldrich (catalog numbers (#) I6657 and #M1404, respectively). H-ICZ and Sotrastaurin were purchased from Cayman Chemical (#22576 and #16726, respectively) and DNS from Santa Cruz Biotechnology (#sc-202592). The chemical drugs PRR851 and PRR846 were synthetized by one of our labs as described^{38 (link)}. All drugs were diluted in dimethyl sulfoxide (DMSO; VWR International) and used at a final concentration of 10 µM for ICZ, H-ICZ, PRR851, and PRR846, 80 µM for DNS, or 1 µM for nocodazole. λ-phosphatase was purchased from Sigma-Aldrich (#P69614) at a stock solution of 400,000 units (U) per ml.