The cDNA was prepared using an iScript gDNA Clear cDNA Synthesis Kit (BioRad). Quantitative RT-PCR was performed using RT2 SYBR Green ROX qPCR Master Mix kit (Cat#330522, Qiagen) for Oct4 exon 1 primers or SensiFAST™ SYBR NO-ROX Mix (Bioline) for 18s RNA and lncRNAs. The qRT-PCR primers for Oct4 exon1 and 18s RNA were previously described (Cherepanova et al., 2016 (link)). For lncRNAs, we used primers as ENSMUST00000140952 (F: CACCCCAGCCCGGTAAAC R: CATGGGCGATCCACATGAA) and ENSMUST00000173605 (F: TTCCTTCTGCGTCAGTATCATCTT R: CCAAGCTGCTTTGCTCAAATT).
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