Profiling Cellular Signaling Pathways

All the cell culture reagents were from HyClone (Logan, UT, USA). Antibodies against Raptor (#2280S), p-Raptor (#2083S), p70S6K1 (#2708S), p-p70S6K1 (#9206S), p-4E-BP1 (#13443S), Rictor (#2114), p-Rictor (#3806S), p-GSK3β (#9336L), p-S6 (#4858S), N-cadherin (#14215S), Slug (#9585S), caspase 3 (#14220S), p21 (#2947), CDK4 (#12,790), CDK6 (#13,331), p-Rb (#9308S) were from Cell Signaling Technology. Antibodies against GSK3β (22104-1-AP) and caspase 9 (10380-1-AP) were obtained from Proteintech. Antibodies specific for S6 (sc-74,459), vimentin (sc-32,322), PARP-1 (sc-8007), Rb (sc-74,562) and Cyclin D1 (sc-20,044) were procured from Santa cruz biotechnology. Antibody against β-actin (A1978) was supplied by Sigma. Z86 was obtained as previously reported [13 (link)].

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Nie S., Chang L., Huang Y., Zhou H., Yang Q., Kong L, & Li Y. (2024). β-carboline derivative Z86 attenuates colorectal cancer cell proliferation and migration by directly targeting PI3K. Natural Products and Bioprospecting, 14(1), 3.

Publication 2024

p-Raptor p70S6K1 p-p70S6K1 p-4E-BP1 Rictor p-Rictor p-GSK3β N-cadherin caspase 3 PARP-1 Cyclin D1 Antibody against β-actin (

Corresponding Organization :

Other organizations : Yunnan University

Top 5 similar protocols

Variable analysis

dependent variables

Raptor
P-Raptor
P70S6K1
P-p70S6K1
P-4E-BP1
Rictor
P-Rictor
P-GSK3β
N-cadherin
Caspase 3
GSK3β
Caspase 9
Vimentin
PARP-1
Cyclin D1
β-actin

control variables

Cell culture reagents from HyClone (Logan, UT, USA)
Antibodies from Cell Signaling Technology, Proteintech, Santa Cruz Biotechnology, and Sigma

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