Cytogenetic Analysis of Ps. libanotica and Ps. tauri

The fixation of the material and the preparation of cytological preparations from the root meristems were performed in accordance with the methodology presented in the article [80 (link)]. The probes were localized on Ps. libanotica and Ps. tauri chromosomes using fluorescent in situ hybridization (FISH) according to the protocol published in [81 (link)]. Detection was carried out using sreptavidin-Cy3 (Vector Laboratories, Peterborough, UK) and Anti-dig-FITC (Roche, Basel, Germany). After the hybridization of the probes, chromosomes were stained with DAPI in the Vectashield medium (Vector Laboratories, Peterborough, UK). The signals were visualized using a DFC 9000 GTC fluorescence microscope (Leica Camera, Wetzlar, Germany) and further processed in Adobe Photoshop (Adobe, Inc., San Jose, CA, USA).