Whole Genome Analysis of ePNT Blastocysts

Clumps of cells were harvested from ePNT blastocysts for whole genome amplification followed by microarray-CGH analysis according to a previously validated protocol using 24Sure Cytochip (Illumina, Cambridge, UK). Cells were obtained from the TE, ICM or both. Lysis and whole genome amplification was performed using the SurePlex kit (Illumina) according to the manufacturer’s instructions. Samples from ePNT blastocysts were labelled with Cy3 while a commercially available reference 46,XY DNA was labelled with Cy5 (Illumina)32 (link). A laser scanner (InnoScan 710, Innopsys, Carbonne, France) was used to analyse the microarrays after washing and drying. The resulting images were analysed using BlueFuse Multi analysis software (Illumina).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Hyslop L.A., Blakeley P., Craven L., Richardson J., Fogarty N.M., Fragouli E., Lamb M., Wamaitha S.E., Prathalingam N., Zhang Q., O’Keefe H., Takeda Y., Arizzi L., Alfarawati S., Tuppen H.A., Irving L., Kalleas D., Choudhary M., Wells D., Murdoch A.P., Turnbull D.M., Niakan K.K, & Herbert M. (2016). Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease. Nature, 534(7607), 383-386.

Publication 2016

24Sure Cytochip SurePlex kit InnoScan 710 BlueFuse Multi analysis software

Blastocysts Cells Genome Microarrays

Corresponding Organization :

Other organizations : Wellcome Centre for Mitochondrial Research, Centre for Life, The Francis Crick Institute, Newcastle University, Oxford Fertility, John Radcliffe Hospital, University of Oxford

Top 5 similar protocols

Variable analysis

independent variables

Cell source (TE, ICM, or both)

dependent variables

Copy number variations (CNVs) detected by microarray-CGH analysis

control variables

Commercially available reference 46,XY DNA

controls

Positive control: Commercially available reference 46,XY DNA
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!