Comparative Metabolomic Analysis of Pigments

Unbiased metabolomics analysis was performed as previously described using an ultra-performance liquid chromatography (UPLC) system (Waters, Milford, USA) [41 (link)]. The scan range in MS and MS/MS modes included 50–1200 m/z. Data acquisition and analysis were controlled by Waters UNIFI V1.71 software. The metabolic profile of the TLC-purified pigment from the transformed S. cerevisiae was compared with that of the purified phleichrome from C. phlei obtained in our previous studies [5 (link), 38 (link), 39 (link)].

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Gwon Y., So K.K., Chun J, & Kim D.H. (2024). Metabolic engineering of Saccharomyces cerevisiae for the biosynthesis of a fungal pigment from the phytopathogenic fungus Cladosporium phlei. Journal of Biological Engineering, 18, 33.

Publication 2024

UPLC) system UNIFI V1.71 software

Corresponding Organization :

Other organizations : Jeonbuk National University

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Variable analysis

independent variables

Transformed S. cerevisiae strain

dependent variables

Metabolic profile of the TLC-purified pigment from the transformed S. cerevisiae
Metabolic profile of the purified phleichrome from C. phlei

control variables

Ultra-performance liquid chromatography (UPLC) system
Scan range in MS and MS/MS modes (50–1200 m/z)
Data acquisition and analysis controlled by Waters UNIFI V1.71 software

controls

Purified phleichrome from C. phlei as a positive control

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