Visualizing Isoeugenol's Effect on Aspergillus Biofilm

The biofilm of A. fumigatus was cultured in a 12-well polystyrene plate at calculated IC₅₀ of isoeugenol to analyse its effect (Manavathu et al. 2014 (link)). To check biofilm eradication property of isoeugenol, biofilm was fully grown on 12-well polystyrene plate for 48 h and then, treated with IC₅₀ of isoeugenol for another 24 h (Hoda et al. 2019 (link); Sav et al. 2018 (link)). To visualise the effect of isoeugenol, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) were performed and samples were processed as described by González-Ramírez et al (2016 (link)), with minor modifications. The A. fumigatus biofilm topology of isoeugenol treated and control (untreated) were viewed under SEM (Zeiss SEM, MA EVO -18 Special Edition). For CLSM, samples were stained with calcofluor white M2R (Sigma, St. Louis, MO, USA) and viewed under Nikon Instruments A1 Confocal Laser Microscope Series with NIS elements C software, Japan.