To compare the microscopic structure of internodes in WT and mutant plants, the eighth internodes of the stems of 40-day-old plants of TopMark and M406 were fixed, rinsed, postfixed, washed, dehydrated, and embedded. Semithin sections were prepared with Formvar-coated gold grids and observed with an Olympus-BX53 light microscope as previously described46 (link).
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