Lucifer yellow CH (Sigma, St. Louis, MO, USA) was selected from a series of fluorescent tracers [5 (link),6 (link)], including dextran-tetramethylrhodamine (Invitrogen, Carlsbad, CA, USA), acridine orange (Sigma), and Gd-DO3A-ethylthiourea-FITC, for in vivo measurements. The Leica TCS SP8 MP FLIM new merging function can store m × n single images during scanning. The whole image of the sample of a designated area can then be obtained by image reconstruction after scanning. Experimental fluorescence images were collected with the LSCM Merging function [6 (link),9 ].
Wang R., Han H., Shi K., Alberts I.L., Rominger A., Yang C., Yan J., Cui D., Peng Y., He Q., Gao Y., Lian J., Yang S., Liu H., Yang J., Wong C., Wei X., Yin F., Jia Y., Tong H., Liu B, & Lei J. (2021). The Alteration of Brain Interstitial Fluid Drainage with Myelination Development. Aging and Disease, 12(7), 1729-1740.
Corresponding Organization : Peking University Third Hospital
Other organizations :
Technical University of Munich, University of Bern, Beijing Children’s Hospital, Capital Medical University, Institute of Applied Physics and Computational Mathematics, Aerospace Center Hospital, Chinese PLA General Hospital
Dextran-tetramethylrhodamine (Invitrogen, Carlsbad, CA, USA)
Acridine orange (Sigma)
Gd-DO3A-ethylthiourea-FITC
controls
Positive controls not explicitly mentioned.
Negative controls not explicitly mentioned.
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