Hydroethanolic extracts and aqueous infusions of P. avium by-products were prepared according to the method previously described [13 (link)]. Firstly, leaves, stems, and flowers were reduced in a dried powder. For the hydroethanolic extracts, 1 g of the dried powder of each sample was dissolved in ethanol/water (50:50, v/v), and posteriorly sonicated for 30 min. After that, the hydroethanolic solutions were shaken at room temperature for 2 h and then again sonicated for 30 min. Finally, the extracts were filtered using a 0.45 µm membrane (Millipore, Bedford, MA, USA) in a vacuum system, evaporated under reduced pressure, freeze-dried, and stored at −20 °C until further use.
Regarding to aqueous infusions, 1 g of dried powder of each sample was subjected to an infusion (100 mL of water) at 100 °C for 3 min, according to the manufacturer’s instructions for daily herbal infusions. Then, infusions were filtered as described above to hydroethanolic extracts, freeze-dried, and stored at −20 °C until further analysis. The extraction yields were reported in a previous work [13 (link)].
Regarding to aqueous infusions, 1 g of dried powder of each sample was subjected to an infusion (100 mL of water) at 100 °C for 3 min, according to the manufacturer’s instructions for daily herbal infusions. Then, infusions were filtered as described above to hydroethanolic extracts, freeze-dried, and stored at −20 °C until further analysis. The extraction yields were reported in a previous work [13 (link)].
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