Computational simulations of Tf-initiated thrombin generation and of resupply reactions were constructed as described previously [8 (link)]. Predicted thrombin concentrations are given at time points to match empirical sampling, which is usually performed at 1 minute intervals. The time to 10 nM total thrombin is used as a computational representation of clot time [11 (link)].
Anticoagulants were modeled by adding the appropriate sets of equations describing their activities to the existing framework of differential equations (see Table 1). The rate constants employed represent average values reflecting literature and in house measurement: Fpx reaction with AT [12 (link)]; Fpx-AT complex reactions with fXa [12 (link)–14 (link)], (in house); with fIXa [15 (link)]; with meizothrombin [16 (link)]; with fXa-fVa complex [13 (link),14 (link)]; and thrombin [12 (link),14 (link)], (in house); Rivaroxaban reaction with fXa [17 (link)], and the fXa-fVa complex [17 (link)].