Strains and plasmids used in the study are listed in Table 1. Strains were cultured in Lysogeny low-salt broth (LB) (EMD Millipore, Burlington, MA) or brain heart infusion broth (BHI; Difco, Sparks, MD) at indicated temperatures with or without kanamycin (Kn), 50 μg mL or chloramphenicol (Cm), 30 μg mL. Congo red agar (67 (link)) was used to confirm pigmentation phenotype of the Y. pestis KIM6+ strains.
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