Cytokine and Epigenomic Profiling in Alzheimer's Disease

Supernatants from PBMCs culture systems after incubation with LPS or PHA, and plasma samples were used for cytokine detection. To explore the effects on the balance between immune response and tolerance, cellular and humoral immunity in AD, concentrations of PBMCs cytokines (IL-1α, IL-2, IL-6, IL-10, TNF-α, and IFN-γ) were determined by sandwich enzyme-linked immunosorbent assay using a commercially available kit (Beijing 4A Biotech Co., Ltd. China). All subjects were measured three times on the same day.
PBMCs pellets from peripheral blood were used to detect genome-wide methylation and hydroxymethylation with MethylFlash Methylated DNA Quantification Kit (Epigentek, Farmingdale, NY) and operated according to the instructions.