To further evaluate the applicability and validity of the established calibration model, it is necessary to conduct external validation using a set of independent samples. For achieving the stable and effective prediction of vitamin C, 40 independent citrus peel samples were randomly collected from fresh harvested citrus fruit and used to validate the best optimized PLS model externally. The operation of the external validation was executed in software Unscramble 10.3X (CAMO, Oslo, Norway).
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