Cell Line Cultivation and Validation

Caco2 (27 (link)) and Calu3 (28 (link)) cell lines were obtained from ATCC and grown in DMEM (Dulbecco's Modified Eagle's medium) with 10% FBS (fetal bovine serum). For all experiments with Caco2 cells they were harvested 48 h post-confluence, a time at which CFTR expression is close to maximum levels (29 (link)). Human bronchial epithelial (HBE) cells were donated by Dr Scott Randell (UNC) and cultured in Bronchial Epithelial Cell Growth Medium, Lonza. Adult human epididymis cells (caput) were described previously (30 (link)). Skin fibroblasts (GM08333) from Coriell Institute were grown in Ham's F-10 media supplement with 15% FBS.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Yang R., Kerschner J.L., Gosalia N., Neems D., Gorsic L.K., Safi A., Crawford G.E., Kosak S.T., Leir S.H, & Harris A. (2015). Differential contribution of cis-regulatory elements to higher order chromatin structure and expression of the CFTR locus. Nucleic Acids Research, 44(7), 3082-3094.

Publication 2015

Bronchial Epithelial Cell Growth Medium GM08333

Adult human Bronchial Caco2 cells Caput Cell lines Cells Cftr Cultured medium Epididymis Epithelial cell Fibroblasts Human Skin Supplement

Corresponding Organization :

Other organizations : Northwestern University, Duke University

Top 5 similar protocols

Variable analysis

independent variables

Cell line (Caco2, Calu3, HBE, adult human epididymis cells, skin fibroblasts)

dependent variables

CFTR expression

control variables

Culture media (DMEM with 10% FBS, Bronchial Epithelial Cell Growth Medium, Ham's F-10 with 15% FBS)
Caco2 cell confluence (48 h post-confluence)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!