Isolation and Identification of E. coli from Clinical Samples

A total of 110 non-duplicated E. coli clinical isolates were collected from 110 different patients in various departments (gastroenterology, endocrinology, neurosurgery, and other wards) at the First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, China from 2015 to 2016. Isolates were identified using standard laboratory methods and the ATB New system (bioMérieux, Lyons, France). Each isolate was further verified by PCR amplification of a 369-bp internal control region from the E. coli marker gene alr [90 (link)]. All strains were stored at − 80 °C and bacteria were grown on MacConkey Agar (Oxoid, Hampshire, UK).

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Li D., Shen M., Xu Y., Liu C., Wang W., Wu J., Luo X., Jia X, & Ma Y. (2018). Virulence gene profiles and molecular genetic characteristics of diarrheagenic Escherichia coli from a hospital in western China. Gut Pathogens, 10, 35.

Publication 2018

ATB New system MacConkey Agar

Agar Bacteria E coli Endocrinology Gene Neurosurgery Patients Strains

Corresponding Organization :

Other organizations : Sichuan University, Chengdu Medical College, First Affiliated Hospital of Chengdu Medical College

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Identification of non-duplicated E. coli clinical isolates

control variables

Collection of 110 non-duplicated E. coli clinical isolates from 110 different patients in various departments (gastroenterology, endocrinology, neurosurgery, and other wards) at the First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, China from 2015 to 2016
Identification of isolates using standard laboratory methods and the ATB New system (bioMérieux, Lyons, France)
Verification of each isolate by PCR amplification of a 369-bp internal control region from the E. coli marker gene alr
Storage of all strains at − 80 °C
Growth of bacteria on MacConkey Agar (Oxoid, Hampshire, UK)

positive controls

PCR amplification of a 369-bp internal control region from the E. coli marker gene alr

negative controls

None explicitly mentioned

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