ChIPs were performed as previously described [17 (link)]. 4 μg of the following antibodies were added to each IP and incubated overnight at 4°C: anti-NF-YA (Santa Cruz, sc-10779), anti-p53 (Santa Cruz, sc-126) and anti-IgG (Santa Cruz, sc-2027), used as control for non-specific interactions. Immunoprecipitated DNA was resuspended in TE buffer, and Real Time PCR analyses were performed with the following primers:
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