Microarray raw data was pre-processed using Robust Multi-array Averaging
(RMA)[17 (link)] and normalized using
quantile normalization. Probes were annotated using manufacturer’s
annotation files (Affymetrix MTA 1.0). Differential expression between
experimental groups was assessed using two-tailed t-test and false discovery
rate correction was performed using Benjamini-Hochberg method. Enrichment of
Gene Ontology terms was done using hypergeometric test and enrichment of
transcription factors was done using oPOSSUM [18 (link)].
(RMA)[17 (link)] and normalized using
quantile normalization. Probes were annotated using manufacturer’s
annotation files (Affymetrix MTA 1.0). Differential expression between
experimental groups was assessed using two-tailed t-test and false discovery
rate correction was performed using Benjamini-Hochberg method. Enrichment of
Gene Ontology terms was done using hypergeometric test and enrichment of
transcription factors was done using oPOSSUM [18 (link)].
