Mouse Ba/F3 cells transfected with human FLT3-ITD (Ba/F3-ITD) and wild-type FLT3 (FLT3-WT) and the FLT3-ITD-expressing MV4-11 human AML cell line were obtained and cultured as previously described (20 (link)). The FLT3-WT cell lines OCI-AML2 and THP-1 were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Braunschweig, GER. Mycoplasma testing was performed every six months with the Mycoplasma Pcr Detection Kit (GeneCopoeia, Rockville, MD).
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Generation and Characterization of FLT3-ITD Cell Lines
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Corresponding Organization :
Other organizations : University of Maryland, Baltimore, University of Michigan–Ann Arbor
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Variable analysis
independent variables
- Cell lines transfected with human FLT3-ITD (Ba/F3-ITD) and wild-type FLT3 (FLT3-WT)
- FLT3-ITD-expressing MV4-11 human AML cell line
- Measured outcomes (not explicitly mentioned)
- FLT3-WT cell lines OCI-AML2 and THP-1 obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Braunschweig, GER
- Mycoplasma testing performed every six months with the Mycoplasma Pcr Detection Kit (GeneCopoeia, Rockville, MD)
- Positive control: Not specified
- Negative control: Not specified
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