Sciatic Nerve Injury and Repair Evaluation

As previously described (Britt et al., 2010 (link); Bittner et al., 2012 (link); Riley et al., 2015 (link)), we first confirmed that extracellularly recorded action potentials (compound action potentials: CAPs) in intact rat sciatic nerves conducted across any potential lesion site in vivo using stimulating and recording tungsten hook electrodes placed on or under the sciatic nerve (Lore et al., 1999 (link); Britt et al., 2010 (link); Bittner et al., 2012 (link); Riley et al., 2015 (link)). Complete crush- or cut-severances were confirmed by an inability to record CAP conduction through the severance site. Sciatic nerves were typically stimulated within minutes after PEG-fusion to determine if CAPs conducted through the lesion site. Stimulations and recordings were made with a Powerlab 4/30 data acquisition system (ADInstruments, Colorado Springs, CO).

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Ghergherehchi C.L., Bittner G.D., Hastings R.L., Mikesh M., Riley D.C., Trevino R.C., Schallert T., Thayer W.P., Sunkesula S.R., Ha T.A., Munoz N., Pyarali M., Bansal A., Poon A.D., Mazal A.T., Smith T.A., Wong N.S, & Dunne P.J. (2016). Effects of extracellular calcium and surgical techniques on restoration of axonal continuity by PEG-fusion following complete cut- or crush-severance of rat sciatic nerves. Journal of neuroscience research, 94(3), 231-245.

Publication 2015

Powerlab 4/30 data

Action potentials Conduction Sciatic nerves Springs Tungsten

Corresponding Organization : The University of Texas at Austin

Other organizations : Texas A&M University, Vanderbilt University

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Protocol cited in 4 other protocols

Variable analysis

independent variables

Potential lesion site

dependent variables

Compound action potentials (CAPs) conduction through the lesion site

control variables

Stimulating and recording tungsten hook electrodes placed on or under the sciatic nerve
Time of stimulation after PEG-fusion (typically within minutes)

controls

Positive control: Intact sciatic nerves with confirmed CAPs conduction across potential lesion site
Negative control: Complete crush- or cut-severances with inability to record CAP conduction through the severance site

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