Lung Tissue Homogenization and Cytokine Analysis

After collecting BALF, remaining lung tissue was resected and homogenized using a tissue homogenizer (Biospec Products, Bartlesville, OK, USA) in lysis buffer and protease inhibitor solution (Sigma-Aldrich, St Louis, MO, USA). After incubation and centrifugation, supernatants were harvested and passed through a 0.45-micron filter (Gelman Science, Ann Arbor, MI, USA). The final preparations were stored at − 20℃ for cytokine analysis as described previously [17 (link)].

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Choi Y.J., Han H., Lee J.H., Lee J., Kim C.Y., Byun M.K., Cho J.H, & Park H.J. (2023). Particulate matter10-induced airway inflammation and fibrosis can be regulated by chitinase-1 suppression. Respiratory Research, 24, 85.

Publication 2023

tissue homogenizer protease inhibitor solution

Buffer Centrifugation Cytokine Lung Protease inhibitor Tissue

Corresponding Organization :

Other organizations : Gangnam Severance Hospital, Yonsei University

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Variable analysis

independent variables

Tissue homogenization using a tissue homogenizer (Biospec Products, Bartlesville, OK, USA)

dependent variables

Cytokine analysis

control variables

Lysis buffer and protease inhibitor solution (Sigma-Aldrich, St Louis, MO, USA)
Incubation and centrifugation
Filtration through a 0.45-micron filter (Gelman Science, Ann Arbor, MI, USA)
Storage at -20°C

controls

No positive or negative controls were explicitly mentioned.

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