ERGs were recorded as previously described (Williamson et al., 2010 (link)). In brief, female flies were immobilized with nontoxic Glue-All (Elmer’s). Recording and reference electrodes containing 2 M NaCl were placed on the fly’s corneal surface and inserted into the thorax, respectively. Voltage measurements were filtered through an electrometer (IE-210; Warner Instruments), digitized with a Digidata 1440A and MiniDigi 1B system (Molecular Devices), and recorded using Clampex (version 10.2; Axon Instruments). 1-s light pulses were provided by a computer-controlled white light-emitting diode system (MC1500; Schott). Eight ERG traces from at least eight individual flies of each genotype were used for quantification with Clampfit software (Axon Instruments).
Protocol detail
Electroretinogram Recording in Flies
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Other organizations : The University of Texas Southwestern Medical Center
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Variable analysis
independent variables
- Genotype of female flies
- Electroretinogram (ERG) traces
- Quantified ERG traces
- Female flies
- Recording and reference electrodes containing 2 M NaCl
- Electrometer (IE-210; Warner Instruments) for voltage measurements
- Digidata 1440A and MiniDigi 1B system (Molecular Devices) for digitization
- Clampex (version 10.2; Axon Instruments) for recording
- 1-s light pulses provided by a computer-controlled white light-emitting diode system (MC1500; Schott)
- Not explicitly mentioned
- Not explicitly mentioned
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