NSCLC Cell Lines Treated with PCAIs

All NSCLC cell lines; NCI-H1299, NCI-H1563, NCI-H460 and A549 were purchased from ATCC (Manassas, VA). The cell lines H1299, H1563 and H460 were maintained in RPMI-1640 medium with 10% heat-inactivated fetal bovine serum (FBS) and antibiotics; the A549 cell line was maintained in F-12K medium with 10% heat-inactivated fetal bovine serum and antibiotics. All cell lines were treated in base media (RPMI-1640 or F-12K) containing 5% FBS which is referred to here as treatment or experimental media. The PCAIs, NSL-BA-040, NSL-BA055, and their non-farnesylated analog NSL-100 were used for treatment. The chemical structures are as previously described [26 (link)].