The separation of nuclear and cytoplasmic proteins was conducted using the nuclear protein and cytoplasmic protein extraction kit (Beyotime, P0027) according to the instructions. It is required to add 1% phase phosphatase inhibitor and 1% protease inhibitor when employing reagents A and B. WB was used to examine the acquired protein samples.
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Nuclear-Cytoplasmic Protein Extraction
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Corresponding Organization : Tongji University
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Variable analysis
independent variables
- Separation of nuclear and cytoplasmic proteins
- Acquired protein samples
- Reagents A and B
- Addition of 1% phase phosphatase inhibitor
- Addition of 1% protease inhibitor
- Positive control: Not specified
- Negative control: Not specified
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