The type strain of T. forsythia (ATCC 43037 = FDC 338) was obtained from ATCC (Manassas, VA, USA) and grown under anaerobic conditions in brain–heart infusion broth with supplements as described previously [12 (link)]. Bacterial DNA was extracted using the GeneElute Bacterial Genomic DNA Kit (Sigma-Aldrich, Vienna, Austria) following the manufacturer’s protocol. The quality of the genomic DNA was checked on a 0.6% standard agarose gel stained with ethidium bromide, and using a NanoDrop ND-1000 spectrophotometer (ThermoFisher, Waltham, MA, USA). Quantification was performed using a Qubit 3.0. fluorometer together with a dsDNA BR assay kit (ThermoFisher, Waltham, MA, USA).
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