Immunohistochemical Analysis of EHMT2 in Colon Tumor
Paraffin-embedded sections of a colon tumor tissue array (T8235722–2, Biochain) were processed in a microwave (90 °C) with antigen-retrieval solution (pH 9) (S2367; Dako), treated with a peroxidase-blocking reagent, and then treated with a protein-blocking reagent (K130, X0909; Dako). The tissue sections were incubated with rabbit anti-EHMT2 antibody (68851; CST) followed by incubation with an HRP-conjugated secondary antibody (Dako). Immunoreactivity was visualized using a chromogenic substrate (Liquid DAB Chromogen; Dako). Finally, the tissue specimens were stained with Mayer’s hematoxylin solution (Hematoxylin QS; Vector Laboratories) for 5 s to discriminate the nucleus from the cytoplasm [22 (link)].
Ryu T.Y., Kim K., Han T.S., Lee M.O., Lee J., Choi J., Jung K.B., Jeong E.J., An D.M., Jung C.R., Lim J.H., Jung J., Park K., Lee M.S., Kim M.Y., Oh S.J., Hur K., Hamamoto R., Park D.S., Kim D.S., Son M.Y, & Cho H.S. (2022). Human gut-microbiome-derived propionate coordinates proteasomal degradation via HECTD2 upregulation to target EHMT2 in colorectal cancer. The ISME Journal, 16(5), 1205-1221.
A protein Anti antibody Antibody Antigen Chromogen Chromogenic substrateCytoplasm Embedded paraffinHematoxylin Microwave NucleusPeroxidase Rabbit Tissue Tumor of colon Vector
Corresponding Organization :
Other organizations :
Korea Research Institute of Bioscience and Biotechnology, Korea Advanced Institute of Science and Technology, Korea University of Science and Technology, Asan Medical Center, University of Ulsan, Kyungpook National University, National Cancer Centre Japan
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