Analytical Methods for Greenhouse Gases

Gas samples (0.1 mL) were regularly taken from headspace of incubations with a pressure-lock precision analytical syringe (Baton Rouge, LA, United States). The concentrations of CH₄ and CO₂ were analyzed using gas chromatographs GC-7890 (Agilent Technologies, United States) equipped with a thermal conductivity detector (Fu et al., 2018 (link)). Liquid samples (0.5 mL) were taken with sterile syringes and centrifuged for 15 min at 17,949 ×g at 4°C. The supernatant was collected, passed through 0.22-μm-pore-size filters, and analyzed for the concentrations of acetate and butyrate with an HPLC-1200 using a Zorbax SB-AQ C₁₈ column (Agilent Technologies, United States) (Fu et al., 2018 (link)). Redox potential (ORP) were measured with an Unisense redox microelectrode RD 100 (Unisense, Denmark).

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Fu L., Zhou T., Wang J., You L., Lu Y., Yu L, & Zhou S. (2019). NanoFe3O4 as Solid Electron Shuttles to Accelerate Acetotrophic Methanogenesis by Methanosarcina barkeri. Frontiers in Microbiology, 10, 388.

Publication 2019

GC-7890 Zorbax SB-AQ C18 column

Acetate Butyrate Gas chromatographs Hplc Microelectrode Pressure Redox Sterile Syringes

Corresponding Organization : Fujian Agriculture and Forestry University

Other organizations : Peking University

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Variable analysis

independent variables

Incubation time

dependent variables

Concentration of CH4 and CO2 in the headspace
Concentration of acetate and butyrate in the liquid samples
Redox potential (ORP)

control variables

Volume of gas samples (0.1 mL)
Volume of liquid samples (0.5 mL)
Centrifugation conditions (15 min at 17,949 ×g at 4°C)
Filtration of liquid samples (0.22-μm-pore-size filters)
Temperature (not explicitly mentioned but can be assumed to be controlled)

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