Metabolomic Analysis of Fungal Mutants

Sample preparation was carried out as above for three biological replicates of each wild type, Δnog1 and ΔcitC mutants. Metabolite profiling was conducted using Ion cyclotron resonance Fourier transform Mass spectrometry (ICR-FT/MS) on a Bruker solariX equipped with a 12 T magnet (Bruker Daltonics, Bremen). Putative metabolites were annotated using the MassTRIX webserver [34 (link)]. Statistical analysis was carried out in MS Excel 2010 and Genedata Expressionist for MS 7.6 (Genedata, Martinsried) using Welch’s T test (p ≤ 0.05) [35 , 36 (link)].

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Fellner L., Simon S., Scherling C., Witting M., Schober S., Polte C., Schmitt-Kopplin P., Keim D.A., Scherer S, & Neuhaus K. (2015). Evidence for the recent origin of a bacterial protein-coding, overlapping orphan gene by evolutionary overprinting. BMC Evolutionary Biology, 15, 283.

Publication 2015

12 T magnet

Biological Cyclotron Mass spectrometry Resonance

Corresponding Organization :

Other organizations : Technical University of Munich, University of Konstanz, Helmholtz Zentrum München, Universität Ulm

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Variable analysis

independent variables

Wild type
Δnog1 mutant
ΔcitC mutant

dependent variables

Metabolite profiling using Ion cyclotron resonance Fourier transform Mass spectrometry (ICR-FT/MS)

control variables

Three biological replicates for each sample type

controls

No positive or negative controls were explicitly mentioned.

Annotations

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