The eluted N-linked glycopeptides were deglycosylated by overnight incubation with 1000 Units of PNGase F (Glycerol-Free, New England Biolabs) as described previously [13 (link)]. Then the samples were desalted by using MicroSpin Columns, 5–200 µL loading volume (The Nest Group, Inc.; Southborough, MA, USA). Samples were centrifugally concentrated to dryness and resuspended in 40 µL 0.1 % FA.
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