Thyroid Cell Lines and Hydrogen Sulfide Treatment

Human normal thyroid cell line Nthy-ori3-1 and human thyroid carcinoma cell lines TPC-1, TT, and ARO were obtained from CoBioer Biosciences Co. Ltd. (Nanjing, Jiangsu, China). Cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin, and 100 μg/ml streptomycin. Cells were grown at 37°C in a humidified atmosphere of 95% air and 5% CO₂ [29 (link)]. Confluent cells were starved overnight in a serum-free RPMI 1640 medium. The cells were then treated with 10, 25, 50, 100, and 200 μM NaHS (an H₂S donor, Sigma-Aldrich, St. Louis, MO, USA) for 24 h. The phosphate-buffered saline (PBS) group was served as a control.