MRI scans of all animal brains were obtained after tumour implantation to assess the tumour growth using a Bruker Pharmascan 7T small animal MRI (Bruker Biospin MRI GmnH, Ettingen, Germany). Axial T1- and T2-weighted images were obtained as previously described [10 (link)]. The tumour volumes at treatment and follow-up MRI were calculated in Gamma Plan (Elekta Instrument AB, Stockholm, Sweden).
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