Culturing Primary Tumor Cells for Drug Screening

Primary cultures of patient tumors were established as previously reported [13 (link)]. Tumor sample collection was approved by the local ethics committee (Kantonale Ethikkommission Zürich, BASEC 2017-00771) and written informed consent was obtained from all subjects prior to tumor sampling. Cells from benign pheochromocytoma and benign adrenal hyperplasia were plated at 250,000 cells /well in the spherical plate 5D (Kugelmeiers, Erlenbach, Switzerland). NCI-H295R, MUC-1 and ACC115m primary cells were cultured as previously described [13 (link), 18 (link)] and cultivated in Sphericalplates 5D for either 7 or 14 days at the densities of 75,000 cells/well, 150,000 cells/well and 75,000 cells/well respectively. The 7 day spheroids were treated with 650 nM Adavosertib (MedChemExpress) for 72 h. Additionally, ACC115m cells were cultivated in Sphericalplates 5D for 7 days and subsequently treated with 25 µM Gemcitabine and 40 µM Cisplatin (both Teva Pharma) for 72 h.