Under anesthesia (100 mg/kg ketamine and 10 mg/kg xylazine, intraperitoneally), mice were injected intra-articularly through the patellar tendon into each knee with the retrograde tracer, fast blue (FB, 1.5 μL 2% in 0.9% saline, Polysciences) or into the left knee with 7.5 μL CFA (10 mg/mL, Chondrex). Vernier calipers were used to measure knee width (as before13 (link)), both before and 24 hours after CFA injection.
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