Rhesus Embryonic and Induced Pluripotent Stem Cells

WT Rhesus ESCs and transgenic Rhesus HD iPSCs were previously established [90 (link)]. Transgenic HD iPSCs expressed both exon 1 of the human HTT gene with 65 CAG repeats and GFP under the control of the human polyubiquitin-C (UBC) promoter [88 (link), 90 (link)]. Rhesus PSCs were cultured on mouse fetal fibroblast (MFF) feeder cells in ESC culture media [Knockout-Dulbecco’s modified Eagle’s medium (KO-DMEM; Invitrogen) with 20% Knockout Serum Replacement (KSR; Invitrogen), 1 mM glutamine, 1 nonessential amino acids (NEAA), and 4 ng/mL of human basic fibroblast growth factor (bFGF; Chemicon, Inc., Tumecula, CA)]. In addition, Rhesus PSC cultures were expanded by mechanical passaging. Since these Rhesus PSC lines were previously used to derive stable NPC cultures [89 (link), 137 (link), 138 (link)], PSCs were collected for experimental analysis, but not further differentiated into NPCs in this study.

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Goodnight A.V., Kremsky I., Khampang S., Jung Y.H., Billingsley J.M., Bosinger S.E., Corces V.G, & Chan A.W. (2019). Chromatin accessibility and transcription dynamics during in vitro astrocyte differentiation of Huntington’s Disease Monkey pluripotent stem cells. Epigenetics & Chromatin, 12, 67.

Publication 2019

Knockout Serum Replacement (KSR;

Amino acids Eagle Escs Exon Feeder cells Fetal Fibroblast Glutamine Human Human fibroblast growth factor 4 Ipscs Mouse Polyubiquitin Pscs Rhesus Serum Transgenic

Corresponding Organization :

Other organizations : Emory University

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Variable analysis

independent variables

Presence of exon 1 of the human HTT gene with 65 CAG repeats and GFP under the control of the human polyubiquitin-C (UBC) promoter in transgenic HD iPSCs

dependent variables

Not explicitly mentioned

control variables

WT Rhesus ESCs
Culture conditions (mouse fetal fibroblast (MFF) feeder cells, ESC culture media [Knockout-Dulbecco's modified Eagle's medium (KO-DMEM; Invitrogen) with 20% Knockout Serum Replacement (KSR; Invitrogen), 1 mM glutamine, 1 nonessential amino acids (NEAA), and 4 ng/mL of human basic fibroblast growth factor (bFGF; Chemicon, Inc., Tumecula, CA)], and mechanical passaging)

controls

Positive control: WT Rhesus ESCs
Negative control: Not mentioned

Annotations

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