Human SAECs, primary (AECIIs) isolated from the mouse lungs and MLE-12
were seeded in 96-well microplates containing 100 μL of culture medium.
Cells were first exposed to bleomycin (10mU/ml) diluted in PBS or vehicle
control (PBS) for 4 hours and then were treated with T3 (15ng/ml) or vehicle
control for 8 hours. The levels of two mitochondrial proteins were measured
simultaneously in each well by using a colorimetric ELISA-kit, according to
manufacturer’s protocol (MitoBiogenesis™ In-Cell ELISA Kit-
Abcam-ab110217)17 (link). The
two proteins are each subunits of a different oxidative phosphorylation enzyme
complex, one protein being subunit I of Complex IV (Cytochrome c oxidase
subunit-COX-IV), which is mitochondrial-DNA-encoded, and Succinate Dehydrogenase
Complex Flavoprotein subunit A-SDHA) which is a 70kDa subunit of Complex II and
nuclear-DNA-encoded.
were seeded in 96-well microplates containing 100 μL of culture medium.
Cells were first exposed to bleomycin (10mU/ml) diluted in PBS or vehicle
control (PBS) for 4 hours and then were treated with T3 (15ng/ml) or vehicle
control for 8 hours. The levels of two mitochondrial proteins were measured
simultaneously in each well by using a colorimetric ELISA-kit, according to
manufacturer’s protocol (MitoBiogenesis™ In-Cell ELISA Kit-
Abcam-ab110217)17 (link). The
two proteins are each subunits of a different oxidative phosphorylation enzyme
complex, one protein being subunit I of Complex IV (Cytochrome c oxidase
subunit-COX-IV), which is mitochondrial-DNA-encoded, and Succinate Dehydrogenase
Complex Flavoprotein subunit A-SDHA) which is a 70kDa subunit of Complex II and
nuclear-DNA-encoded.
