Quantitative Histelide for Alzheimer's Pathology

We quantified regional concentration of Aβ₄₂ (Covance Research Products, SIG-39320, Clone 6E10, Mouse monoclonal 1:4000) and Phospho-τ (Thermo Scientific, MN1020, Clone AT8, Mouse monoclonal 1:100) in middle frontal gyrus (MFG), superior and middle temporal gyri (SMTG), and inferior parietal lobule (IPL) exactly according to a method pioneered in our laboratory to obtain quantitative molecular data from formalin-fixed paraffin-embedded tissue that we call Histelide, for “histology and ELISA on a glass slide” [21 (link)]; antibody specificity is reported in Table 2 of this publication [21 (link)]. We have shown previously in the same autopsy brain regions that Histelide has a linear concentration response, and that regions of brain from control cases that lacked phospho-τ or Aβ₄₂ deposition did not produce signal above noise [21 (link)]. Furthermore, Histelide measurements of phospho-τ correlate with Braak stage for neurofibrillary degeneration and Histelide measurements of Aβ₄₂ correlate with CERAD NP score [22 (link)]. Individual Histelide measures were missing on 4%–6% of the 420 autopsies because of depleted or missing tissue blocks. All available data points were used.