Total RNA-seq libraries were prepared using the SMART-Seq Stranded Kit (Clontech) following the manufacturer’s instructions. Poly(A) RNA-seq libraries were prepared as previously described (56 (link)) using a SMARTer ultralow input RNA cDNA preparation kit (Clontech). A Nextera XT DNA library preparation kit (Illumina) was used for poly(A) RNA-seq cDNA fragmentation, adaptor ligation, and amplification according to the manufacturer’s instructions. The prepared RNA-seq libraries were sequenced on NextSeq 550 (Illumina) with paired-ended 75-bp reads.
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Total RNA-seq and Poly(A) RNA-seq Library Preparation
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Corresponding Organization : Harvard University
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- Total RNA-seq libraries were prepared using the SMART-Seq Stranded Kit (Clontech) following the manufacturer's instructions.
- Poly(A) RNA-seq libraries were prepared as previously described (56 (link)) using a SMARTer ultralow input RNA cDNA preparation kit (Clontech).
- A Nextera XT DNA library preparation kit (Illumina) was used for poly(A) RNA-seq cDNA fragmentation, adaptor ligation, and amplification according to the manufacturer's instructions.
- The prepared RNA-seq libraries were sequenced on NextSeq 550 (Illumina) with paired-ended 75-bp reads.
- Manufacturer's instructions were followed for the SMART-Seq Stranded Kit (Clontech) and the Nextera XT DNA library preparation kit (Illumina).
- No positive or negative controls were explicitly mentioned in the provided information.
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